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Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO) (HF879036)

Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)
Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)
Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)
Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)
Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)
Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)
Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)
Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)
Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)
Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)
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  • 概要

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概要
カタログ番号HF879036
説明
Infliximab (HF879036) is a research-grade recombinant antibody targeting CD2. Produced in mammalian cells with native-like glycosylation.
Highlights
  • ●Research Grade — For PK/PD studies, assay development, and ADA research.
  • ●Native Glycosylation — Mammalian expression ensures native-like patterns.
種反応性Human
アプリケーションELISA, Bioactivity: FCM, Functional assay, Research in vivo
宿主種Chimeric
クローン性Monoclonal
アイソタイプIgG1, kappa
テスト済みアプリケーション FCM
ターゲット C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form
エンドトキシンレベル < 10 EU/mg
純度 >95% as determined by SDS-PAGE.
精製 Protein A/G purified from cell culture supernatant.
アクセッション番号 P01375
形態 Liquid
保存バッファー 0.01M PBS pH 7.4.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名TA-650, cA2, 170277-31-3
背景Onercept is a recombinant soluble human tumor necrosis factor-alpha p55 receptor for use in Crohn's disease research.
注意事項 For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
イメージ
  • Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)

    SEC-HPLC

    SEC-HPLC detection for Research Grade Infliximab.

  • Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)

    Bioactivity

    Detects Human TNFa/TNF-alpha in indirect ELISAs.

  • Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)

    SDS-PAGE

    SDS-PAGE for Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)

  • Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)

    Flow CytoMetry

    Flow-cytometry PE using anti-human TNFa antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human TNFa monoclonal antibody (Catalog: HF879036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)

    Flow CytoMetry

    Flow-cytometry using anti-human TNFa antibody. 293T Transtected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog: HF879036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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