Anti-Human PSCA Reference Antibody (Mk-4721, RUO) (HT361016)

Name: Anti-Human PSCA Reference Antibody (Mk-4721, RUO)
Brand: abinScience
SKU: HT361016
Price: 176 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HT361016
Reactivité des espèces	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG1-kappa
Activité biologique	AGS-1C4D4 displays potent ADCC activity against PSCA-expressing HPAC and Panc0203 pancreatic cell lines, with activity greatly reduced by deglycosylation, and is more potent than the hybridoma-derived AGS-PSCA antibody in this assay [1]. AGS-1C4D4 exhibits CDC activity against PSCA-expressing recombinant B300.19 cells, with activity greatly reduced by deglycosylation, and is equally effective as the hybridoma-derived AGS-PSCA antibody in this assay [1]. AGS-1C4D4 binds to PSCA with a K d of 0.2 nM, displaying similar binding affinity to the hybridoma-derived AGS-PSCA antibody [1]. AGS-1C4D4 significantly inhibits established pancreatic tumor growth and metastasis in orthotopic HPAC xenograft models when combined with Gemcitabine (), and also significantly inhibits pancreatic tumor formation, local invasion, and distant metastasis in the same orthotopic xenograft model [1].
Système d'expression	Mammalian cells
Applications testées	FCM
Cible	Prostate stem cell antigen, PSCA
Niveau d'endotoxines	< 10 EU/mg
Pureté	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	O43653
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	AGS-PSCA, MK-4721
Contexte	Prostate stem cell antigen (PSCA) is a ~11 kDa protein. May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro.
Reférences	1. Tony Navas, et al. Abstract 4566: AGS-1C4D4: A fully human anti-PSCA antibody inhibits tumor formation and metastasis in orthotopic models of pancreatic cancer. Cancer Res (2011) 71 (8_Supplement): 4566. [HT361016]
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Images

Bioactivity

SEC-HPLC detection for Research Grade MK-4721.
SDS-PAGE

SDS-PAGE for Research Grade MK-4721.
Flow cytometry

Flow-cytometry using anti-human PSCA antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PSCA monoclonal antibody (Catalog HT361016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.