Anti-Human PDGF R alpha Reference Antibody (Olaratumab, RUO) (HB927016)

Name: Anti-Human PDGF R alpha Reference Antibody (Olaratumab, RUO)
Brand: abinScience
SKU: HB927016
Price: 176 USD
Availability: InStock

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100ug
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25mg
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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер	HB927016
Описание	Olaratumab (HB927016) is a research-grade recombinant antibody targeting CD140. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Реактивность видов	Human
Применения	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG1-kappa
Система экспрессии	Mammalian cells
Проверенные приложения	FCM
Цель	PDGFR2, Platelet-derived growth factor receptor alpha, RHEPDGFRA, Alpha platelet-derived growth factor receptor, PDGFR-2, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGF-R-alpha, Alpha-type platelet-derived growth factor receptor, CD140a antigen, CD140 antigen-like family member A, PDGFRA, PDGFR-alpha, CD140a
Уровень эндотоксинов	< 10 EU/mg
Чистота	>95% purity as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P16234
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	3G3, IMC-3G3, LY3012207, 1024603-93-7
Фон	Platelet-derived growth factor receptor alpha (CD140a/PDGFRA) is a ~122 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi.
Примечание	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Изображения

SEC-HPLC

SEC-HPLC detection for Research Grade Olaratumab.
Bioactivity

Detects CD140a/PDGFRA in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human PDGFRA antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PDGFRA monoclonal antibody (Catalog HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Research Grade Olaratumab
Flow CytoMetry

Flow-cytometry using FITC anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using APC anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.