Anti-Human ALCAM/CD166 Reference Antibody (Praluzatamab, RUO) (HC678016)

Name: Anti-Human ALCAM/CD166 Reference Antibody (Praluzatamab, RUO)
Brand: abinScience
SKU: HC678016
Price: 176 USD
Availability: InStock

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100ug
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25mg
50mg
100mg

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер	HC678016
Описание	Praluzatamab (HC678016) is a research-grade recombinant antibody targeting CD166. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Реактивность видов	Human
Применения	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG1-kappa
Система экспрессии	Mammalian cells
Цель	Activated leukocyte cell adhesion molecule, MEMD, CD166 antigen, ALCAM, CD166
Уровень эндотоксинов	< 10 EU/mg
Чистота	>95% purity as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	Q13740
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	CX-2009, 2145109-70-0
Фон	CD166 antigen (ALCAM) is a ~65 kDa protein. Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM. Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation. 1. Hassan, NJ. et al. (2004) European journal of immunology 34, 930-40. PMID: 15048703 2. Ikeda, K. et al. (2004) The Journal of biological chemistry 279, 55315-23. PMID: 15496415 3. Zimmerman, AW. et al. (2006) Blood 107, 3212-20. PMID: 16352806 4. Iolyeva, M. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 978-90. PMID: 23169771 5. Escoda-Ferran, C. et al. (2014) FEBS letters 588, 2805-13. PMID: 24945728 6. Bowen, MA. et al. (1995) The Journal of experimental medicine 181, 2213-20. PMID: 7760007 7. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938 8. Xu, Z. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 1207-1219. PMID: 29263213 9. Chitteti, BR. et al. (2014) Blood 124, 519-29. PMID: 24740813
Примечание	For research use only. Not suitable for clinical or therapeutic use.

Изображения

Bioactivity

Detects CD166/ALCAM in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Praluzatamab.
Flow CytoMetry

Flow-cytometry using APC anti-human CD166 antibody. SH-SY5Y cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD166 monoclonal antibody (Catalog: HC678016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD166 antibody. SH-SY5Y cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD166 monoclonal antibody (Catalog: HC678016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD166 antibody. SH-SY5Y cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD166 monoclonal antibody (Catalog: HC678016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD166 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD166 monoclonal antibody (Catalog: HC678016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.