Home > Products > Antibodies > Flow Cytometry (FACS) Antibodies

Anti-Human CD166/ALCAM Recombinant Antibody (SAA0066), APC (HC678137)

Datasheet Share product Copy info

Anti-Human CD166/ALCAM Recombinant Antibody (SAA0066), APC

Anti-Human CD166/ALCAM Recombinant Antibody (SAA0066), APC

Price(USD): $

Spec:

50T
100T

Number:

Contact us

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HC678137
Description	Anti-Human CD166/ALCAM Antibody (SAA0066), APC [SAA0066] (HC678137) is a mouse monoclonal antibody detecting ALCAM in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	SAA0066
Conjugation	APC
Clonality	Monoclonal
Target	Activated leukocyte cell adhesion molecule, MEMD, CD166 antigen, ALCAM, CD166
Endotoxin level	Please contact with the lab for this information.
Accession	Q13740
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	CD166 antigen (ALCAM) is a ~65 kDa protein. Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM. Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation. 1. Hassan, NJ. et al. (2004) European journal of immunology 34, 930-40. PMID: 15048703 2. Ikeda, K. et al. (2004) The Journal of biological chemistry 279, 55315-23. PMID: 15496415 3. Zimmerman, AW. et al. (2006) Blood 107, 3212-20. PMID: 16352806 4. Iolyeva, M. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 978-90. PMID: 23169771 5. Escoda-Ferran, C. et al. (2014) FEBS letters 588, 2805-13. PMID: 24945728 6. Bowen, MA. et al. (1995) The Journal of experimental medicine 181, 2213-20. PMID: 7760007 7. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938 8. Xu, Z. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 1207-1219. PMID: 29263213 9. Chitteti, BR. et al. (2014) Blood 124, 519-29. PMID: 24740813
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using APC anti-human CD166 antibody. SH-SY5Y cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD166 monoclonal antibody (Catalog: HC678137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD166/ALCAM Recombinant Antibody (SAA0066), APC.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

=

Concentration

×

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

×

V₁ (Stock Vol.)

=

Working Solution

C₂ (Working Conc.)

×

V₂ (Working Vol.)

Recommendation

Contact us for custom quotes, bulk requests and any other issues.

Mail: support@abinScience.com

Distributor list

Anti-Human CD166/ALCAM Recombinant Antibody (SAA0066), APC