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| カタログ番号 | HW755026 |
|---|---|
| 説明 |
Prasinezumab (HW755026) is a research-grade recombinant antibody targeting Alpha-synuclein. Produced in mammalian cells with native-like glycosylation.
Highlights
|
| 種反応性 | Human |
| アプリケーション | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| 宿主種 | Human |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1-kappa |
| 発現システム | Mammalian cells |
| テスト済みアプリケーション | WB |
| ターゲット | Alpha-synuclein, PARK1, Non-A4 component of amyloid precursor, NACP, Non-A beta component of AD amyloid, SNCA |
| エンドトキシンレベル | < 10 EU/mg |
| 純度 | >95% purity as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P37840 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | PRX002, RG-7935, 1960462-19-4 |
| 背景 | Alpha-synuclein (SNCA) is a ~14 kDa protein. Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. 1. Burré, J. et al. (2010) Science (New York, N.Y.) 329, 1663-7. PMID: 20798282 2. Butler, B. et al. (2015) The Journal of biological chemistry 290, 29542-54. PMID: 26442590 3. Logan, T. et al. (2017) Nature neuroscience 20, 681-689. PMID: 28288128 4. Huang, CC. et al. (2018) Journal of cell science 131. PMID: 30404828 |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10) (HV080407) Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
SDS-PAGE for Anti-Human alpha-Synuclein Reference Antibody (Prasinezumab, RUO)
SEC-HPLC detection for Research Grade Prasinezumab.
Detects SNCA in indirect ELISAs.
Western blot analysis was performed using anti-SNCA monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human SNCA (Catalog No: HW755012), 50ng
Lane 2: recombinant human SNCA (Catalog No: HW755012), 30ng
Lane 3: recombinant human SNCA (Catalog No: HW755012), 20ng
Lane 4: recombinant human SNCA (Catalog No: HW755012), 10ng
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