Research Grade Anti-Human SNCA/Alpha-synuclein (Lu AF82422) (HW755086)

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100ug
1mg

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Datasheet

Overview

Catalog No.	HW755086
Description	Anti-Human SNCA/Alpha-synuclein (Lu AF82422) (HW755086) is a research-grade recombinant antibody targeting Alpha-synuclein. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1
Clone ID	Lu AF82422
Expression system	Mammalian Cells
Clonality	Monoclonal
Target	Alpha-synuclein, PARK1, Non-A4 component of amyloid precursor, NACP, Non-A beta component of AD amyloid, SNCA
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P37840
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names	LuAF82422
Background	Alpha-synuclein (SNCA) is a ~14 kDa protein. Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. 1. Burré, J. et al. (2010) Science (New York, N.Y.) 329, 1663-7. PMID: 20798282 2. Butler, B. et al. (2015) The Journal of biological chemistry 290, 29542-54. PMID: 26442590 3. Logan, T. et al. (2017) Nature neuroscience 20, 681-689. PMID: 28288128 4. Huang, CC. et al. (2018) Journal of cell science 131. PMID: 30404828
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human SNCA/Alpha-synuclein (Lu AF82422).
Western blot

Western blot analysis was performed using anti-SNCA monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human SNCA (Catalog No: HW755012)
Lane 2: negative control
Bioactivity

Detects recombinant human SNCA (Catalog No: HW755012) in indirect ELISA