中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HC598016 |
|---|---|
| 説明 |
Anti-Human CD161 Reference Antibody (Rezorstobart, RUO) (HC598016) is a human monoclonal antibody detecting CD161. Expressed in Mammalian cells.
|
| 種反応性 | Human |
| アプリケーション | ELISA, Bioactivity: FCM, Functional assay, Research in vivo |
| 宿主種 | Human |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1-kappa |
| 生物学的活性 | CD161 has K i s of 8.2 nM and 1.4 nM for BRD4 BD1 and BRD4 BD2, respectively [1]. CD161 (30-3000 nM; 1 hours) is very effective in inducing rapid down-regulation of c-Myc at as early as the 1 h time point and in a dose-dependent manner [1]. Western Blot Analysis [1] Cell Line: MV4;11 leukemia cells. Concentration: 30, 100, 300, 1000, 3000 nM Incubation Time: 1 hours Result: Induced rapid down-regulation of c-Myc at as early as the 1 hours time point and in a dose-dependent manner. CD161 (po; 20, 40 mg/kg/day; 45 days) achieves essentially complete tumor growth inhibition [1]. CD161 (5 mg/kg (iv), 25 mg/kg (po); 0-24 hours) has the t 1/2 of 2.4 hours (iv) and 2.9 hours (po) for rat; the C max of 7333 ng/mL (po) for rat. The t 1/2 of mice is 0.5 hours (iv) and 1.60 hours (po); the C max of mice is 983.1 ng/mL (po) [1]. |
| 発現システム | Mammalian cells |
| 性質 | Recombinant |
| ターゲット | CLEC5B, KLRB1, CD161, HNKR-P1a, C-type lectin domain family 5 member B, NKRP1A, Natural killer cell surface protein P1A, Killer cell lectin-like receptor subfamily B member 1, NKR-P1A |
| エンドトキシンレベル | < 10 EU/mg as determined by LAL method. |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | Q12918 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | 2762201-86-3 |
| 背景 | Killer cell lectin-like receptor subfamily B member 1 (CD161/KLRB1) is a ~25 kDa protein. Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Also binds to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. |
| 参考文献 | 1. Zhao Y, et al. Structure-Based Discovery of 4-(6-Methoxy-2-methyl-4-(quinolin-4-yl)-9H-pyrimido[4,5-b]indol-7-yl)-3,5-dimethylisoxazole (CD161) as a Potent and Orally Bioavailable BET Bromodomain Inhibitor. J Med Chem. 2017 May 11;60(9):3887-3901. [HC598016] |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
SDS-PAGE for Research Grade Rezorstobart
Detects recombinant human CD161 (Catalog No: HC598021) in indirect ELISA
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
