TEV Protease (VK816012)

Name: TEV Protease
Brand: abinScience
SKU: VK816012
Price: 198 USD
Availability: InStock

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Número de catálogo	VK816012
Descripción	TEV Protease (VK816012) expressed in E. coli. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Longitud de proteína	TEV Protease is cloned from Tobacco etch virus (TEV) and expressed in E.coli.
Aplicaciones	TEV Protease is a highly specific cysteine protease. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ↓(S/G/A/M/C/H).
Actividad biológica	One unit of activity is defined as the amount of enzyme required to cleave >85% of 50 μg of substrate after incubation at 30°C for 1 h in 1× TEV Buffer (50 mM Tris, pH 8.0, 0.5 mM EDTA, and 1 mM DTT).
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Forma	Liquid
Buffer de almacenamiento	25 mM Tris-HCl, pH8.0, 50 mM NaCl, 1 mM EDTA, 50% glycerol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	TEV Protease, Genome polyprotein
Nota	For research use only.