Lumpy Skin Disease Virus (LSDV) Research Reagents — Recombinant Proteins

P32 / LSDV074 — Immunodominant Antigen
P32 (also known as the major envelope protein homolog of VACV H3L) is the most immunodominant LSDV antigen and the primary target for serological ELISA-based LSD diagnostics. Anti-P32 antibodies appear early in infection and are highly abundant in convalescent sera, making P32-based ELISA the most widely used serological assay for LSD surveillance. Available: P32 recombinant protein N-His (VK817012).

IMV Envelope Protein / LSDV060
LSDV060 is the IMV (intracellular mature virus) surface protein — homolog of VACV D8L. IMV antigens are targets for virus-neutralizing antibodies that block primary infection. Available in two formats: C-His (VK756011) and N-His (VK756012).

EEV Glycoprotein / LSDV122 & Host Range Protein / LSDV141
• LSDV122 (EEV glycoprotein, homolog of VACV B5R): EEV surface protein that protects extracellular virions from complement — targets for antibodies that block virus dissemination. Available: C-His (VK607011) and N-His (VK607012)
• LSDV141 (EEV host range protein): determines host range and is involved in EEV formation. Available: C-His (VK610011) and N-His (VK610012)

RPO30 / LSDV035 — DIVA Diagnostic Marker
RPO30 is a 30 kDa RNA polymerase subunit that is the basis for RPO30 PCR-based DIVA assays — distinguishing wild-type LSDV from Neethling-based homologous vaccines (which have RPO30 deletions/mutations). Recombinant RPO30 protein can serve as a positive control for RPO30-based diagnostic development. Available: RPO30 recombinant protein N-His (VK792012).

Fusion Protein / LSDV117 & GPCR / LSDV011
• LSDV117 (fusion protein): involved in cell-to-cell spread — recombinant protein N-His (VK684012)
• LSDV011 (G-protein-coupled chemokine-like receptor): viral GPCR that modulates host immune signaling — a unique immune evasion tool found in capripoxviruses. Available: recombinant protein N-His (VK811012)

Which protein is best for LSD serological ELISA?
P32/LSDV074 (VK817012) is the standard choice — P32 is the most immunodominant LSDV antigen with the highest sensitivity and specificity in published ELISA validation studies. Use as coating antigen for indirect ELISA to detect anti-LSDV IgG in cattle sera.

How do I implement DIVA diagnostics for LSDV?
DIVA (Differentiating Infected from Vaccinated Animals) for LSDV uses RPO30-based PCR — Neethling homologous vaccine strains have characteristic RPO30 gene deletions that distinguish them from wild-type LSDV. Our RPO30 protein (VK792012) serves as a positive control for assay validation. For serological DIVA, combining P32 ELISA (detects both infected and vaccinated) with EEV-specific antigens (LSDV122/LSDV141) may provide differentiation — contact us for DIVA assay design support.

Do LSDV reagents cross-react with sheeppox and goatpox viruses?
LSDV, sheeppox virus (SPPV), and goatpox virus (GTPV) are all capripoxviruses sharing >96% nucleotide identity. P32, LSDV060, and LSDV122 homologs are highly conserved across all three viruses — cross-reactivity is expected and can be advantageous for pan-capripoxvirus diagnostics. Species-specific differentiation requires PCR-based approaches targeting variable regions (RPO30, GPCR).

Explore reagents for related poxviruses and veterinary pathogens:

LSDV ✓ Mpox / MPXV Vaccinia (VACV) ASFV RVFV NDV