Часто задаваемые вопросы(FAQ)

UniProt is a comprehensive protein database collaboratively maintained by EMBL-EBI, SIB, and NCBI. It comprises UniProtKB, Proteomes, UniRef, and UniParc, offering extensive protein sequences, functional annotations, and structural data. With integrated tools like BLAST and Align, UniProt supports advanced research in bioinformatics, molecular biology, and proteomics.

Learn how to choose the best isotype control antibody to ensure accurate in vivo research. Explore essential selection criteria, their role in experimental validation, and Abinscience’s high-quality, low-endotoxin antibody solutions.

Learn about polyethylene glycol (PEG), its properties, uses, and detection using anti-PEG antibodies. Find answers to common questions on PEG-related products.

Clone numbers identify specific hybridoma cell lines in monoclonal antibody production, ensuring consistency and traceability. Find them in the 'overview' tab of the product datasheet.

How to find the optimal antibody concentration for WB, IHC, IF, ELISA, and flow cytometry. Learn titration strategies, starting dilution recommendations, and common mistakes that cause weak signal or high background.

Step-by-step nuclear/cytoplasmic fractionation protocol for WB: hypotonic lysis method, Buffer A/C recipes, NP-40 optimization, fraction purity markers (Lamin B1, α-Tubulin), and troubleshooting guide. Covers p-STAT, p-Smad, YAP, p-ERK, NF-κB, β-catenin nuclear translocation detection.

Research reagents for CD47/SIRPa studies. Covers the 'don't eat me' signal mechanism, therapeutic targeting strategies, and available antibodies, proteins, and biosimilars.

A practical guide to antibody storage and handling. Covers storage temperatures for different antibody formats, aliquoting strategies, the role of sodium azide and carrier proteins, signs of antibody degradation, and tips for maximizing antibody performance over time.

A practical guide to understanding and verifying antibody specificity. Covers common causes of cross-reactivity, the five pillars of antibody validation, species reactivity interpretation, and a step-by-step checklist for confirming antibody performance in your specific experimental system.

A practical guide to neutralization assays for research antibodies. Covers the principle of antibody-mediated neutralization, common assay formats (PRNT, pseudovirus, cell-based reporter, receptor blocking ELISA), antibody selection for neutralization studies, and tips for optimizing dose-response curves.