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Обзор
Каталожный номерHY259053
Описание
Anti-Human Fibronectin Reference Antibody (F8) (HY259053) is a human monoclonal antibody detecting Fibronectin in ELISA. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
Реактивность видовHuman
ПримененияELISA
Хозяинский видHuman
КлоональностьMonoclonal
ИзотипIgG1, kappa
ID клонаF8
Цель Extra Domain A, ED-A, Anastellin, CIG, Cold-insoluble globulin, FN, FN1, Fibronectin, Ugl-Y1, Ugl-Y2, Ugl-Y3
Уровень эндотоксинов < 10 EU/mg
Чистота >95% as determined by SDS-PAGE.
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P02751
Форма Liquid
Буфер хранения 0.01M PBS pH 7.4.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Информация об использовании продукта
Применение Разбавление
ELISA 1:2000-1:20000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Fibronectin (EDB) is a ~272 kDa protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation.

1. Owens, RJ. et al. (1986) The EMBO journal 5, 2825-30. PMID: 3024962
2. Garcia-Pardo, A. et al. (1987) The Biochemical journal 241, 923-8. PMID: 3593230
3. Calaycay, J. et al. (1985) The Journal of biological chemistry 260, 12136-41. PMID: 3900070
4. Rostagno, A. et al. (1994) The Journal of biological chemistry 269, 31938-45. PMID: 7989369
5. Su, MT. et al. (2021) International immunology 33, 447-458. PMID: 34089617
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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