Anti-Human Fibronectin Reference Antibody (HuBC-1-hIL12, RUO) (HY259026)

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Spec:

100ug
1mg

Number:

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Overview

Catalog No.

HY259026

Description

Anti-Human FN1/Fibronectin (HuBC-1) (HY259026) is a research-grade recombinant antibody targeting Cold-insoluble globulin. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

Species reactivity

Human

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Host species

Human

Isotype

IgG1-hIL12, kappa

Clone ID

HuBC-1-hIL12

Expression system

Mammalian Cells

Clonality

Monoclonal

Target

EDB, Cold-insoluble globulin, CIG, FN, Fibronectin, FN1

Endotoxin level

< 10 EU/mg

Purity

>95% purity as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P02751

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

HuBC-1ScFv, AS1409, hubc1, bc-1, bc1

Background

Fibronectin (FN) is a ~272 kDa protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation.

1. Owens, RJ. et al. (1986) The EMBO journal 5, 2825-30. PMID: 3024962

2. Garcia-Pardo, A. et al. (1987) The Biochemical journal 241, 923-8. PMID: 3593230

3. Calaycay, J. et al. (1985) The Journal of biological chemistry 260, 12136-41. PMID: 3900070

4. Rostagno, A. et al. (1994) The Journal of biological chemistry 269, 31938-45. PMID: 7989369

5. Su, MT. et al. (2021) International immunology 33, 447-458. PMID: 34089617

Note

For research use only. Not suitable for clinical or therapeutic use.

Images

References

Datasheet

Anti-Human Fibronectin Reference Antibody (HuBC-1-hIL12, RUO).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human Fibronectin Reference Antibody (HuBC-1-hIL12, RUO) (HY259026)

Overview

Images

References

Datasheet

SDS

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