3C Protease (VK794012)

Name: 3C Protease
Brand: abinScience
SKU: VK794012
Price: 298 USD
Availability: InStock

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Datasheet
SDS

Vista general

Número de catálogo	VK794012
Descripción	3C Protease (VK794012) expressed in E. coli. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Longitud de proteína	3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli.
Aplicaciones	Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Forma	Liquid
Buffer de almacenamiento	50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for 3C Protease

Bioactivity

M: Marker
1: Control
2: Fusion protein digest with 3C protease

Case study		Formula
concentration	1 mg/ml	c mg/ml
volume	100 µl	V ml
substrate	100 µg	cV mg
10X Buffer	11 µl	V/9 ml
3C Protease	1 µl	cV/100 ml

Mix the components thoroughly and incubate the reaction at 4°C for 16 hours. After incubation, take 2–10 µg of the control and digested sample for analysis by SDS-PAGE.

Referencias

Datasheet