3C Protease (VK794012)

Name: 3C Protease
Brand: abinScience
SKU: VK794012
Price: 298 USD
Availability: InStock

データシート製品共有情報コピー

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5000U

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	VK794012
説明	3C Protease (VK794012) expressed in E. coli. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
発現システム	E. coli
タンパク質長	3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli.
アプリケーション	Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
性質	Recombinant
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
形態	Liquid
保存バッファー	50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
再構成	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
発送	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
注意事項	For research use only.

イメージ

SDS-PAGE

SDS-PAGE for 3C Protease

Bioactivity

M: Marker
1: Control
2: Fusion protein digest with 3C protease

Case study		Formula
concentration	1 mg/ml	c mg/ml
volume	100 µl	V ml
substrate	100 µg	cV mg
10X Buffer	11 µl	V/9 ml
3C Protease	1 µl	cV/100 ml

Mix the components thoroughly and incubate the reaction at 4°C for 16 hours. After incubation, take 2–10 µg of the control and digested sample for analysis by SDS-PAGE.

参考文献

データシート