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  • 100ug
  • 1mg
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Обзор
Каталожный номерHB191021
Описание
GMP Grade Human HGF (Tag Free) (HB191021) expressed in Mammalian cells. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
  • ●Recombinant — Consistent lot-to-lot performance.
  • ●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
  • ●Bioactive — Verified biological activity.
Система экспрессииMammalian cells
Номер доступаP14210
Длина белкаα chain:Gln32-Arg494 & β chain:Val495-Ser728
ПримененияELISA, Functional assay
ВидыHomo sapiens (Human)
Биологическая активность Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is ≤2.5 ng/ml..
Характер Recombinant
Уровень эндотоксинов <0.01 EU/ug
Чистота >95% as determined by SDS-PAGE.
Форма Lyophilized
Буфер хранения Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияHGF, HPTA, Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A, SF, Scatter factor
Фон

Hepatocyte growth factor (SF,HPTA,Hepatopoietin-A,Hepatocyte growth factor,Scatter factor,HGF) is a ~83 kDa protein. Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.

1. Tolbert, WD. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13264-9. PMID: 20624990
2. Stamos, J. et al. (2004) The EMBO journal 23, 2325-35. PMID: 15167892
3. Hashimoto, T. et al. (2010) The FEBS journal 277, 4888-900. PMID: 20977675
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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