Clostridium Difficile Toxin B/tcdB Antibody Pair Set (JN876908)

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Каталожный номер	JN876908
Описание	Clostridium Difficile Toxin B/tcdB Antibody Pair Set (JN876908) is a capture: mouse, detection: mouse antibody detecting Toxin B/tcdB in ELISA. Suitable for Clostridioides difficile. Highlights ●Detection: Biotin Conjugated — Ready for direct detection without secondary antibody.
Реактивность видов	Clostridioides difficile
Хозяинский вид	Capture: Mouse, Detection: Mouse
Иммуноген	Toxin B/tcdB
Конъюгация	Detection: Biotin
Проверенные приложения	S-ELISA
Цель	Toxin B/tcdB
Специфичность	Toxin B/tcdB
Применения	ELISA
Форма	Liquid
Буфер хранения	Capture: 0.01M PBS pH 7.4; Detection: 0.01M PBS pH 7.4, 50% glycerol Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Тип анализа	Sandwich ELISA
Характеристики	Reagents are sufficient for at least 1 x 96 well plates using recommended protocol.
Фон	Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis. 1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489 2. Xu, H. et al. (2014) Nature 513, 237-41. PMID: 24919149 3. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482 4. Qa'Dan, M. et al. (2000) Infection and immunity 68, 2470-4. PMID: 10768933 5. Barth, H. et al. (2001) The Journal of biological chemistry 276, 10670-6. PMID: 11152463 6. Pfeifer, G. et al. (2003) The Journal of biological chemistry 278, 44535-41. PMID: 12941936 7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356 9. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706
Примечание	For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Clostridium Difficile Toxin B/tcdB Antibody Pair Set.pdf

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Protocol

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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