Human IL-8/CXCL8 Antibody Pair Set (HC517908)

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SDS
Protocol

Обзор

Каталожный номер	HC517908
Описание	Human IL-8/CXCL8 Antibody Pair Set (HC517908) is a capture: mouse, detection: mouse antibody detecting IL-8/CXCL8 in ELISA. Suitable for Human. Highlights ●Detection: Biotin Conjugated — Ready for direct detection without secondary antibody.
Чувствительность	3.57 pg/mL
Диапазон	31.25-2,000 pg/mL
Реактивность видов	Human
Хозяинский вид	Capture: Mouse, Detection: Mouse
Иммуноген	IL-8/CXCL8
Конъюгация	Detection: Biotin
Проверенные приложения	S-ELISA
Цель	IL-8/CXCL8
Специфичность	IL-8/CXCL8
Применения	ELISA
Форма	Liquid
Буфер хранения	Capture: 0.01M PBS pH 7.4; Detection: 0.01M PBS pH 7.4, 50% glycerol Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Тип анализа	Sandwich ELISA
Характеристики	Reagents are sufficient for at least 1 x 96 well plates using recommended protocol.
Фон	Interleukin-8 (IL8/CXCL8) is a ~11 kDa protein. Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways. 1. Zinkernagel, AS. et al. (2008) Cell host & microbe 4, 170-8. PMID: 18692776 2. Hammond, ME. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 155, 1428-33. PMID: 7636208 3. Van Damme, J. et al. (1990) European journal of immunology 20, 2113-8. PMID: 2145175 4. Jacobi, HH. et al. (1998) International archives of allergy and immunology 116, 53-9. PMID: 9623510 5. Holmes, WE. et al. (1991) Science (New York, N.Y.) 253, 1278-80. PMID: 1840701 6. Murphy, PM. et al. (1991) Science (New York, N.Y.) 253, 1280-3. PMID: 1891716 7. Takami, M. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 4559-66. PMID: 11971003 8. Damaj, BB. et al. (1996) The Journal of biological chemistry 271, 12783-9. PMID: 8662698
Примечание	For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Изображения

Standard Curve

Standard Curve of Human IL-8/CXCL8 Antibody Pair Set

Ссылки

Даташит

Human IL-8/CXCL8 Antibody Pair Set.pdf

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Protocol

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа