Mouse IFNG/IFN-gamma Antibody Pair Set (MF813908)

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Обзор
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Protocol

Обзор

Каталожный номер	MF813908
Описание	Mouse IFNG/IFN-gamma Antibody Pair Set (MF813908) is a capture: mouse, detection: mouse antibody detecting IFNG/IFN-gamma in ELISA. Suitable for Mouse. Highlights ●Detection: Biotin Conjugated — Ready for direct detection without secondary antibody.
Чувствительность	4.86 pg/mL
Диапазон	9.375-600 pg/mL
Реактивность видов	Mouse
Хозяинский вид	Capture: Mouse, Detection: Mouse
Иммуноген	IFNG/IFN-gamma
Конъюгация	Detection: Biotin
Проверенные приложения	S-ELISA
Цель	IFNG/IFN-gamma
Специфичность	IFNG/IFN-gamma
Применения	ELISA
Форма	Liquid
Буфер хранения	Capture: 0.01M PBS pH 7.4; Detection: 0.01M PBS pH 7.4, 50% glycerol Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Тип анализа	Sandwich ELISA
Характеристики	Reagents are sufficient for at least 1 x 96 well plates using recommended protocol.
Фон	Interferon gamma (IFNG/IFN-gamma) is a ~19 kDa protein. Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. 1. El Bougrini, J. et al. (2006) Cellular and molecular biology (Noisy-le-Grand, France) 52, 9-15. PMID: 16914093 2. Hisamatsu, H. et al. (1996) The Journal of experimental medicine 183, 1807-16. PMID: 8666937 3. Greenlund, AC. et al. (1993) The Journal of biological chemistry 268, 18103-10. PMID: 8349687 4. Akiyama, K. et al. (1994) FEBS letters 343, 85-8. PMID: 8163024 5. Fabunmi, RP. et al. (2001) Journal of cell science 114, 29-36. PMID: 11112687 6. Lah, TT. et al. (1995) FEBS letters 363, 85-9. PMID: 7729559
Примечание	For research use only

Изображения

Standard Curve

Standard Curve of Mouse IFNG/IFN-gamma Antibody Pair Set

Ссылки

Даташит

Mouse IFNG/IFN-gamma Antibody Pair Set.pdf

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Protocol

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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