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| カタログ番号 | HT232014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human AXIN1 (Ser653-Asp862). | ||||||||
| ターゲット | AXIN, Axin-1, hAxin, Axis inhibition protein 1, AXIN1 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O15169 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Axin-1 (AXIN1) is a ~95 kDa protein. Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. 1. Kusano, S. et al. (2002) Molecular and cellular biology 22, 6393-405. PMID: 12192039 2. Goto, T. et al. (2016) FEBS letters 590, 1291-303. PMID: 27098453 3. Lu, Y. et al. (2017) Cell research 27, 1422-1440. PMID: 28829046 4. Liu, W. et al. (2006) The EMBO journal 25, 1646-58. PMID: 16601693 5. Li, Q. et al. (2007) Cancer research 67, 66-74. PMID: 17210684 | ||||||||
| 注意事項 | For research use only. |
A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting AXIN1 (HT232014) at a concentration of 13.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of AXIN1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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