Human AXIN1 Recombinant Protein (N-His) (HT232012)

Name: Human AXIN1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HT232012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT232012
Description	Recombinant Human AXIN1 Protein, N-His (HT232012) expressed in E. coli, spanning Ser653-Asp862. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O15169
Protein length	Ser653-Asp862
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	26.08 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	AXIN, Axin-1, hAxin, Axis inhibition protein 1, AXIN1
Background	Axin-1 (AXIN1) is a ~95 kDa protein. Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. 1. Kusano, S. et al. (2002) Molecular and cellular biology 22, 6393-405. PMID: 12192039 2. Goto, T. et al. (2016) FEBS letters 590, 1291-303. PMID: 27098453 3. Lu, Y. et al. (2017) Cell research 27, 1422-1440. PMID: 28829046 4. Liu, W. et al. (2006) The EMBO journal 25, 1646-58. PMID: 16601693 5. Li, Q. et al. (2007) Cancer research 67, 66-74. PMID: 17210684
Note	For research use only.

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SDS-PAGE for Recombinant Human AXIN1 Protein, N-His

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HT232012-SDS-PAGE-1.jpg

Datasheet

Human AXIN1 Recombinant Protein (N-His).pdf

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Human AXIN1 Recombinant Protein (N-His) (HT232012)

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