Anti-BCL2 Polyclonal Antibody (HY131014)

Name: Anti-BCL2 Polyclonal Antibody
Brand: abinScience
SKU: HY131014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HY131014

Description

Anti-BCL2 Polyclonal Antibody (HY131014) is a rabbit polyclonal antibody detecting BCL2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Dog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human BCL2 (Met1-Asp211).

Cible

Apoptosis regulator Bcl-2, BCL2

Purification

Purified by antigen affinity column.

Numéro d'accès

P10415

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Apoptosis regulator Bcl-2 (BCL2) is a ~26 kDa protein. Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function.

1. Eguchi, Y. et al. (1992) Nucleic acids research 20, 4187-92. PMID: 1508712

2. Yin, XM. et al. (1994) Nature 369, 321-3. PMID: 8183370

3. Ruvolo, PP. et al. (2001) Leukemia 15, 515-22. PMID: 11368354

4. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871

5. Chen, D. et al. (2010) The Journal of biological chemistry 285, 38214-23. PMID: 20889974

6. Strappazzon, F. et al. (2011) The EMBO journal 30, 1195-208. PMID: 21358617

7. Bruey, JM. et al. (2007) Cell 129, 45-56. PMID: 17418785

Reférences

1. Gracillin Protects Liver Ischemia-Reperfusion Injury from Oxidative Stress-Induced Apoptosis. [HY131014]

Note

For research use only.

Images

Western blot

Gracillin pretreatment significantly restored Bcl-2 expression suppressed by liver ischemia-reperfusion injury.

Reférences

Gracillin Protects Liver Ischemia-Reperfusion Injury from Oxidative Stress-Induced Apoptosis

Fiche technique

Anti-BCL2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-BCL2 Polyclonal Antibody (HY131014)

Aperçu

Images

Reférences

Fiche technique

SDS

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