Anti-Human Bcl2 Recombinant Antibody (SAA1989) (HY131023)

Name: Anti-Human Bcl2 Recombinant Antibody (SAA1989)
Brand: abinScience
SKU: HY131023
Price: 110 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY131023

Species reactivity

Human

Applications

ELISA, FCM

Host species

Mouse

Isotype

VHH-mFc

Clone ID

SAA1989

Clonality

Monoclonal

Target

Apoptosis regulator Bcl-2, BCL2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P10415

RRID

Anti-Human Bcl2 Antibody (SAA1989) (abinScience Cat# HY131023, RRID:AB_3727774)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Apoptosis regulator Bcl-2 (BCL2) is a ~26 kDa protein. Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function.

1. Eguchi, Y. et al. (1992) Nucleic acids research 20, 4187-92. PMID: 1508712

2. Yin, XM. et al. (1994) Nature 369, 321-3. PMID: 8183370

3. Ruvolo, PP. et al. (2001) Leukemia 15, 515-22. PMID: 11368354

4. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871

5. Chen, D. et al. (2010) The Journal of biological chemistry 285, 38214-23. PMID: 20889974

6. Strappazzon, F. et al. (2011) The EMBO journal 30, 1195-208. PMID: 21358617

7. Bruey, JM. et al. (2007) Cell 129, 45-56. PMID: 17418785

Note

For research use only.

Images

Flow CytoMetry

Flow-cytometry using PE anti-human BCL2 antibody. A549 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human BCL2 monoclonal antibody (Catalog: HY131023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human BCL2 antibody. A549 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human BCL2 monoclonal antibody (Catalog: HY131023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.