Anti-Human BCL2 Recombinant Nanobody (SAA1206) (HY131013)

Name: Anti-Human BCL2 Recombinant Nanobody (SAA1206)
Brand: abinScience
SKU: HY131013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY131013

Description

Anti-Human BCL2 Nanobody (SAA1206) [SAA1206] (HY131013) is a alpaca monoclonal antibody detecting BCL2 in ELISA, SPR. Suitable for Human.

Species reactivity

Human

Applications

ELISA, SPR

Host species

Alpaca

Clonality

Monoclonal

Isotype

VHH-8His-Cys-tag

Clone ID

SAA1206

Target

Apoptosis regulator Bcl-2, BCL2

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P10415

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Apoptosis regulator Bcl-2 (BCL2) is a ~26 kDa protein. Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function.

1. Eguchi, Y. et al. (1992) Nucleic acids research 20, 4187-92. PMID: 1508712

2. Yin, XM. et al. (1994) Nature 369, 321-3. PMID: 8183370

3. Ruvolo, PP. et al. (2001) Leukemia 15, 515-22. PMID: 11368354

4. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871

5. Chen, D. et al. (2010) The Journal of biological chemistry 285, 38214-23. PMID: 20889974

6. Strappazzon, F. et al. (2011) The EMBO journal 30, 1195-208. PMID: 21358617

7. Bruey, JM. et al. (2007) Cell 129, 45-56. PMID: 17418785

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human BCL2 Nanobody (SAA1206)

References

Datasheet

Anti-Human BCL2 Recombinant Nanobody (SAA1206).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human BCL2 Recombinant Nanobody (SAA1206) (HY131013)

Overview

Images

References

Datasheet

SDS