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Anti-Integrin alpha 4/CD49d Polyclonal Antibody (HY596014)

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Vista general
Número de catálogoHY596014
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CD49d/ITGA4 (Asn35-Leu315).
Objetivo ITGA4, CD49d, CD49 antigen-like family member D, CD49D, VLA-4 subunit alpha, Integrin alpha-IV, Integrin alpha-4
Purificación Purified by antigen affinity column.
Número de acceso P13612
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Integrin alpha-4 (CD49d/ITGA4) is a ~114 kDa protein. Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1.

1. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415
2. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536
3. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877
4. Morris, GE. et al. (2022) British journal of pharmacology 179, 4958-4973. PMID: 35802072
Nota For research use only.
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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