Anti-Human Integrin alpha 4/CD49d Reference Antibody (Natalizumab, RUO) (HY596016)

Name: Anti-Human Integrin alpha 4/CD49d Reference Antibody (Natalizumab, RUO)
Brand: abinScience
SKU: HY596016
Price: 176 USD
Availability: InStock

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Overview

Catalog No.	HY596016
Description	Natalizumab (HY596016) is a research-grade recombinant antibody targeting CD49. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG4-kappa
Expression system	Mammalian cells
Clonality	Monoclonal
Target	ITGA4, CD49d, CD49 antigen-like family member D, CD49D, VLA-4 subunit alpha, Integrin alpha-IV, Integrin alpha-4
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P13612
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	AN100226, BG00002, 189261-10-7
Background	Integrin alpha-4 (CD49d/ITGA4) is a ~114 kDa protein. Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. 1. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415 2. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536 3. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877 4. Morris, GE. et al. (2022) British journal of pharmacology 179, 4958-4973. PMID: 35802072
Note	For research use only. Not suitable for clinical or therapeutic use.

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Bioactivity

SEC-HPLC detection for Research Grade Natalizumab.
SDS-PAGE

SDS-PAGE for Research Grade Natalizumab
Flow CytoMetry

Flow-cytometry using APC anti-human CD49d antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD49d monoclonal antibody (Catalog: HY596016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD49d antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD49d monoclonal antibody (Catalog: HY596016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD49d antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD49d monoclonal antibody (Catalog: HY596016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human Integrin alpha 4/CD49d Reference Antibody (Natalizumab, RUO).pdf

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Recombinant Proteins

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Anti-Human Integrin alpha 4/CD49d Reference Antibody (Natalizumab, RUO) (HY596016)

Overview

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References

Datasheet

SDS

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