Anti-Human CD49d/ITGA4 Recombinant Antibody (SAA0019) (HY596107)

Name: Anti-Human CD49d/ITGA4 Recombinant Antibody (SAA0019)
Brand: abinScience
SKU: HY596107
Price: 196 USD
Availability: InStock

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Overview

Catalog No.	HY596107
Species reactivity	Human
Applications	ELISA, FCM
Host species	Rabbit
Isotype	IgG, kappa
Clone ID	SAA0019
Clonality	Monoclonal
Target	ITGA4, CD49d, CD49 antigen-like family member D, CD49D, VLA-4 subunit alpha, Integrin alpha-IV, Integrin alpha-4
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P13612
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Integrin alpha-4 (CD49d/ITGA4) is a ~114 kDa protein. Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. 1. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415 2. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536 3. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877 4. Morris, GE. et al. (2022) British journal of pharmacology 179, 4958-4973. PMID: 35802072
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human CD49d antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD49d antibody monoclonal antibody (Catalog # HY596107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # PTB96441) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD49d/ITGA4 Recombinant Antibody (SAA0019).pdf

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Recombinant Proteins

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Anti-Human CD49d/ITGA4 Recombinant Antibody (SAA0019) (HY596107)

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