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Anti-GFP Recombinant Nanobody (SAA0958) (ZP144033)

Anti-GFP Recombinant Nanobody (SAA0958)
Anti-GFP Recombinant Nanobody (SAA0958)
Anti-GFP Recombinant Nanobody (SAA0958)
Anti-GFP Recombinant Nanobody (SAA0958)
Precio(USD):
Especificación:
  • 20ug
  • 50ug
  • 100ug
  • 1mg
Número:
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  • Datasheet

  • SDS

Vista general
Número de catálogoZP144033
Descripción
Anti-GFP Nanobody (SAA0958) [SAA0958] (ZP144033) is a alpaca monoclonal antibody detecting GFP in ELISA. Suitable for Aequorea victoria.
Reactividad de especiesGeneral
AplicacionesELISA, IF
Especie huéspedAlpaca
ClonalidadMonoclonal
IsotipoVHH-8His-Cys-tag
ID de clonSAA0958
Objetivo Green fluorescent protein, GFP
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Purified by Nickel column
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Información de uso del producto
Aplicación Dilución
ELISA 1:2000-1:20000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nota For research use only.
Imágenes
  • Anti-GFP Recombinant Nanobody (SAA0958)

    SDS-PAGE

    SDS-PAGE for Anti-GFP Nanobody (SAA0958)

  • Anti-GFP Recombinant Nanobody (SAA0958)

    Immunofluorescence

    Immunofluorescent analysis of 4% PFA-fixed and 0.1% Triton X-100-permeabilized 293T cells transfected with pEGFP-N1 using VHH anti-GFP antibody (Cat. No. ZP144033, His-tagged) at 20 μg/mL. After incubation with mouse anti-His antibody, CY3-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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