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| カタログ番号 | ZP144033 | ||||
|---|---|---|---|---|---|
| 説明 |
Anti-GFP Nanobody (SAA0958) [SAA0958] (ZP144033) is a alpaca monoclonal antibody detecting GFP in ELISA. Suitable for Aequorea victoria.
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| 種反応性 | General | ||||
| アプリケーション | ELISA, IF | ||||
| 宿主種 | Alpaca | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | VHH-8His-Cys-tag | ||||
| クローンID | SAA0958 | ||||
| ターゲット | Green fluorescent protein, GFP | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Purified by Nickel column | ||||
| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. | ||||
| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| 注意事項 | For research use only. |
SDS-PAGE for Anti-GFP Nanobody (SAA0958)
Immunofluorescent analysis of 4% PFA-fixed and 0.1% Triton X-100-permeabilized 293T cells transfected with pEGFP-N1 using VHH anti-GFP antibody (Cat. No. ZP144033, His-tagged) at 20 μg/mL. After incubation with mouse anti-His antibody, CY3-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
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