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| カタログ番号 | HV675013 | ||||
|---|---|---|---|---|---|
| 種反応性 | Human | ||||
| アプリケーション | Blocking, ELISA, FCM, SPR | ||||
| 宿主種 | Mouse | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG2a, lambda | ||||
| クローンID | 3H9 | ||||
| ターゲット | A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAM-TS 13, ADAM-TS13, ADAMTS-13, 3.4.24.87, von Willebrand factor-cleaving protease, vWF-CP, vWF-cleaving protease, ADAMTS13, C9orf8, UNQ6102/PRO20085 | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | Q76LX8 | ||||
| RRID | Anti-Human ADAMTS13 Antibody (3H9) (abinScience Cat# HV675013, RRID:AB_3725878) |
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| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 注意事項 | For research use only. | ||||
| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |
SDS-PAGE for Anti-Human ADAMTS13 Antibody (3H9).
Flow-cytometry using anti-human ADAMTS13 antibody. ADAMTS13 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ADAMTS13 antibody monoclonal antibody (Catalog # HV675013, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human ADAMTS13 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ADAMTS13 antibody monoclonal antibody (Catalog # HV675013, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC- conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human ADAMTS13 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ADAMTS13 monoclonal antibody (Catalog: HV675013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
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