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Anti-Human ATP5PB Monoclonal Antibody (1A480) (HC151015)

Anti-Human ATP5PB Monoclonal Antibody (1A480)
Anti-Human ATP5PB Monoclonal Antibody (1A480)
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Vista general
Número de catálogoHC151015
Descripción
Anti-Human ATP5PB Monoclonal Antibody (1A480) [1A480] (HC151015) is a mouse monoclonal antibody detecting ATP5PB in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Recombinant — Superior batch-to-batch reproducibility with defined sequences.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG
ID de clon1A480
Inmunógeno E. coli - derived recombinant human ATP5PB (Ser96-Met256).
Objetivo ATP synthase peripheral stalk subunit b mitochondrial, ATP synthase F(0) complex subunit B1 mitochondrial, ATP synthase peripheral stalk-membrane subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATPase subunit b, ATP5PB
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso P24539
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

ATP synthase peripheral stalk subunit b, mitochondrial (ATP5PB) is a ~28 kDa protein. Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256
Nota For research use only.
Imágenes
  • Anti-Human ATP5PB Monoclonal Antibody (1A480)

    SDS-PAGE

    SDS-PAGE for Anti-Human ATP5PB Monoclonal Antibody (1A480)

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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