Anti-Human ATP5PB Monoclonal Antibody (1A481) (HC151025)

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Catalog No.	HC151025
Description	Anti-Human ATP5PB Monoclonal Antibody (1A481) [1A481] (HC151025) is a mouse monoclonal antibody detecting ATP5PB in ELISA, IHC, WB. Suitable for Human. Highlights ●Recombinant — Superior batch-to-batch reproducibility with defined sequences. ●Multi-Application — Validated across multiple applications.
Species reactivity	Human
Applications	ELISA, IHC, WB
Host species	Mouse
Isotype	IgG
Clone ID	1A481
Clonality	Monoclonal
Immunogen	E. coli - derived recombinant human ATP5PB (Ser96-Met256).
Target	ATP synthase peripheral stalk subunit b mitochondrial, ATP synthase F(0) complex subunit B1 mitochondrial, ATP synthase peripheral stalk-membrane subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATPase subunit b, ATP5PB
Purification	Protein A/G purified from cell culture supernatant.
Accession	P24539
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	ATP synthase peripheral stalk subunit b, mitochondrial (ATP5PB) is a ~28 kDa protein. Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. 1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256
Note	For research use only.

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Anti-Human ATP5PB Monoclonal Antibody (1A481).pdf

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Anti-Human ATP5PB Monoclonal Antibody (1A481) (HC151025)

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