Anti-Human BAX Recombinant Antibody (6A7) (HC491013)

Name: Anti-Human BAX Recombinant Antibody (6A7)
Brand: abinScience
SKU: HC491013
Price: 110 USD
Availability: InStock

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Цена(USD):

Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HC491013

Реактивность видов

Human, Mouse

Применения

ELISA, FCM, WB

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG1, kappa

ID клона

6A7

Цель

BAX, Bcl2-L-4, BCL2L4, Bcl-2-like protein 4, Apoptosis regulator BAX

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

Q07812

RRID

Anti-Human BAX Antibody (6A7) (abinScience Cat# HC491013, RRID:AB_3726396)
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Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Примечание

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Изображения

Bioactivity

SEC-HPLC detection for Anti-Human BAX Antibody (6A7).
Flow Cytometry

Flow-cytometry using anti-human BAX antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human BAX antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human BAX antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human BAX Antibody (6A7).
Flow CytoMetry

Flow-cytometry using FITC anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human BAX monoclonal antibody (Catalog: HC491013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human BAX monoclonal antibody (Catalog: HC491013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.