Anti-Human Semaphorin 4D/CD100 Recombinant Antibody (VX15/2503) (HV563107)

Name: Anti-Human Semaphorin 4D/CD100 Recombinant Antibody (VX15/2503)
Brand: abinScience
SKU: HV563107
Price: 191 USD
Availability: InStock

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概要
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データシート
SDS

概要

カタログ番号	HV563107
種反応性	Human
アプリケーション	FCM
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG4, kappa
クローンID	VX15/2503
テスト済みアプリケーション	FCM
ターゲット	A8, Semaphorin-4D, GR3, BB18, SEMAJ, C9orf164, CD100, SEMA4D
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	Q92854
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Semaphorin-4D (CD100/SEMA4D) is a ~96 kDa protein. Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. 1. Janssen, BJ. et al. (2010) Nature 467, 1118-22. PMID: 20877282 2. Vodrazka, P. et al. (2009) The European journal of neuroscience 30, 1193-208. PMID: 19788569 3. Basile, JR. et al. (2005) Molecular and cellular biology 25, 6889-98. PMID: 16055703 4. Hall, KT. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 11780-5. PMID: 8876214
注意事項	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD100/SEMA4D Antibody (VX15/2503).
Flow Cytometry

Flow-cytometry using anti-human CD100 antibody.CD100 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD100 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD100 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects recombinant human CD100/SEMA4D (Catalog No: HV563011) in indirect ELISA.