Anti-Human Semaphorin 4D/CD100 Recombinant Antibody (VX15/2503) (HV563107)

Name: Anti-Human Semaphorin 4D/CD100 Recombinant Antibody (VX15/2503)
Brand: abinScience
SKU: HV563107
Price: 191 USD
Availability: InStock

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Каталожный номер	HV563107
Реактивность видов	Human
Применения	FCM
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG4, kappa
ID клона	VX15/2503
Проверенные приложения	FCM
Цель	A8, Semaphorin-4D, GR3, BB18, SEMAJ, C9orf164, CD100, SEMA4D
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	Q92854
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон	Semaphorin-4D (CD100/SEMA4D) is a ~96 kDa protein. Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. 1. Janssen, BJ. et al. (2010) Nature 467, 1118-22. PMID: 20877282 2. Vodrazka, P. et al. (2009) The European journal of neuroscience 30, 1193-208. PMID: 19788569 3. Basile, JR. et al. (2005) Molecular and cellular biology 25, 6889-98. PMID: 16055703 4. Hall, KT. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 11780-5. PMID: 8876214
Примечание	For research use only.

Изображения

Bioactivity

SEC-HPLC detection for Anti-Human CD100/SEMA4D Antibody (VX15/2503).
Flow Cytometry

Flow-cytometry using anti-human CD100 antibody.CD100 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD100 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD100 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects recombinant human CD100/SEMA4D (Catalog No: HV563011) in indirect ELISA.