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Anti-Human IL-5 R alpha/CD125 Polyclonal Antibody (HX243014)

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Aperçu
Numéro de catalogueHX243014
Description
Anti-Human CD125/IL5RA Polyclonal Antibody (HX243014) is a rabbit polyclonal antibody detecting IL5RA in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CD125/IL5RA (Gly42-Cys182).
Cible IL-5R subunit alpha, IL-5 receptor subunit alpha, IL5RA, IL-5RA, IL-5R-alpha, Interleukin-5 receptor subunit alpha, CDw125, CD125, IL5R
Purification Purified by antigen affinity column.
Numéro d'accès Q01344
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Interleukin-5 receptor subunit alpha (CD125/IL5RA) is a ~47 kDa protein. Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Acts by forming a heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5. In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway.

1. Monahan, J. et al. (1997) Journal of immunology (Baltimore, Md. : 1950) 159, 4024-34. PMID: 9378992
2. Tavernier, J. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 7041-5. PMID: 1495999
3. Kusano, S. et al. (2012) Protein science : a publication of the Protein Society 21, 850-64. PMID: 22528658
4. Ogata, N. et al. (1998) Blood 91, 2264-71. PMID: 9516124
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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