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Anti-Human Coagulation factor III/Tissue Factor Recombinant Antibody (SAA0059) (HB058107)

Anti-Human Coagulation factor III/Tissue Factor Recombinant Antibody (SAA0059)
Anti-Human Coagulation factor III/Tissue Factor Recombinant Antibody (SAA0059)
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  • データシート

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概要
カタログ番号HB058107
種反応性Human
アプリケーションFCM
宿主種Human
クローン性Monoclonal
アイソタイプIgG1, kappa
クローンIDSAA0059
テスト済みアプリケーション FCM
ターゲット Tissue factor, CD142, F3, Thromboplastin, Coagulation factor III, TF
エンドトキシンレベル Please contact with the lab for this information.
純度 >95% as determined by SDS-PAGE.
精製 Protein A/G purified from cell culture supernatant.
アクセッション番号 P13726
形態 Liquid
保存バッファー 0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

安定性と保存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Tissue factor (CD142/F3) is a ~33 kDa protein. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. CD142 is the therapeutic target of tisotumab vedotin (Tivdak).

注意事項 For research use only.
イメージ
  • Anti-Human Coagulation factor III/Tissue Factor Recombinant Antibody (SAA0059)

    Flow Cytometry

    Flow-cytometry using anti-human CD142 antibody.CD142 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD142 antibody monoclonal antibody (Catalog # HB058107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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