Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061) (HW664107)

Name: Anti-Human EMMPRIN/CD147 Recombinant Antibody (SAA0061)
Brand: abinScience
SKU: HW664107
Price: 191 USD
Availability: InStock

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概要
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参考文献
データシート
SDS

概要

カタログ番号	HW664107
種反応性	Human
アプリケーション	ELISA, FCM, IHC, WB
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1, kappa
クローンID	SAA0061
テスト済みアプリケーション	WB, FCM
ターゲット	Hepatoma-associated antigen, BSG, HAb18G, 5F7, TCSF, OK blood group antigen, Collagenase stimulatory factor, CD147, Leukocyte activation antigen M6, Basigin, Extracellular matrix metalloproteinase inducer, EMMPRIN, Tumor cell-derived collagenase stimulatory factor
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P35613
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Basigin (CD147/BSG) is a ~42 kDa protein. Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors. In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors. May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes. 1. Aït-Ali, N. et al. (2015) Cell 161, 817-32. PMID: 25957687 2. Redzic, JS. et al. (2011) Journal of molecular biology 411, 68-82. PMID: 21620857
注意事項	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD147/BSG Antibody (ABS1891).
Flow Cytometry

Flow-cytometry using anti-human CD147 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD147 monoclonal antibody (Catalog HW664107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD147/BSG monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD147/BSG (Catalog No: HW664011)