Human CD147/BSG Recombinant Protein (C-His) (HW664011)

Name: Human CD147/BSG Recombinant Protein (C-His)
Brand: abinScience
SKU: HW664011
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	HW664011
Description	Recombinant Human CD147/BSG Protein, C-His (HW664011) expressed in Mammalian Cells, spanning Met1-His205. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	Q865R3
Protein length	Met1-His205
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	23.07 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Hepatoma-associated antigen, BSG, HAb18G, 5F7, TCSF, OK blood group antigen, Collagenase stimulatory factor, CD147, Leukocyte activation antigen M6, Basigin, Extracellular matrix metalloproteinase inducer, EMMPRIN, Tumor cell-derived collagenase stimulatory factor
Background	Basigin (CD147/BSG) is a ~22 kDa protein. Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells. Plays an important role in targeting the monocarboxylate transporters SLC16A1/GLUT1, SLC16A3, SLC16A8, SLC16A11 and SLC16A12 to the plasma membrane. Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling. Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA and KDR/VEGFR2 in endothelial cells. Plays a role in neurite outgrowth.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CD147/BSG Protein, C-His
Bioactivity

Detects Meplazumab in indirect ELISAs.
Bioactivity

Detects Gavilimomab in indirect ELISAs.
Bioactivity

Detects Human CD147/BSG Antibody (4A5) in indirect ELISAs.
Western blot

Detected by Anti-CD147/BSG monoclonal antibody (Catalog No: HW664026)
Western blot

Detected by Anti-CD147/BSG monoclonal antibody (Catalog No: HS720016)
Western blot

Detected by Anti-CD147/BSG monoclonal antibody (Catalog No: HB714016)
Bioactivity

Detected by Ziralimumab (Catalog No: HW664026) in indirect ELISA.

References

HW664011-SDS-PAGE-1.jpg

Datasheet

Human CD147/BSG Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

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Working Solution

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V₂ (Working Vol.)

Recombinant Proteins

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Human CD147/BSG Recombinant Protein (C-His) (HW664011)

Overview

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