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Anti-Human CD157 Recombinant Antibody (A2), FITC (HC654117)

Anti-Human CD157 Recombinant Antibody (A2), FITC
Anti-Human CD157 Recombinant Antibody (A2), FITC
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  • 50T
  • 100T
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Vista general
Número de catálogoHC654117
Reactividad de especiesHuman
AplicacionesFCM
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG1, kappa
ID de clonA2
ConjugaciónFITC
Objetivo ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, Bone marrow stromal antigen 1, BST-1, ADP-ribosyl cyclase 2, CD157, Cyclic ADP-ribose hydrolase 2, BST1, cADPr hydrolase 2
Nivel de endotoxinas < 10 EU/mg
Pureza >90% as determined by SDS-PAGE.
Productos asociados Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), FITC
Número de acceso Q10588
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo

ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2 (CD157/BST1) is a ~35 kDa protein. Catalyzes both the synthesis of cyclic ADP-beta-D-ribose (cADPR) from NAD(+), and its hydrolysis to ADP-D-ribose (ADPR). Cyclic ADPR is known to serve as an endogenous second messenger that elicits calcium release from intracellular stores, and thus regulates the mobilization of intracellular calcium (Probable). May be involved in pre-B-cell growth (Probable).

1. Hirata, Y. et al. (1994) FEBS letters 356, 244-8. PMID: 7805847
Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Imágenes
  • Anti-Human CD157 Recombinant Antibody (A2), FITC

    Flow CytoMetry

    Flow-cytometry using FITC anti-human CD157 antibody. THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD157 monoclonal antibody (Catalog: HC654117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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