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Anti-Human CD157/BST1 Recombinant Antibody (A2), APC (HC654137)

Anti-Human CD157/BST1 Recombinant Antibody (A2), APC
Anti-Human CD157/BST1 Recombinant Antibody (A2), APC
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Overview
Catalog No.HC654137
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG1, kappa
Clone IDA2
ConjugationAPC
Clonality Monoclonal
Target ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, Bone marrow stromal antigen 1, BST-1, ADP-ribosyl cyclase 2, CD157, Cyclic ADP-ribose hydrolase 2, BST1, cADPr hydrolase 2
Endotoxin level Please contact with the lab for this information.
Associated products Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
Accession Q10588
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2 (CD157/BST1) is a ~35 kDa protein. Catalyzes both the synthesis of cyclic ADP-beta-D-ribose (cADPR) from NAD(+), and its hydrolysis to ADP-D-ribose (ADPR). Cyclic ADPR is known to serve as an endogenous second messenger that elicits calcium release from intracellular stores, and thus regulates the mobilization of intracellular calcium (Probable). May be involved in pre-B-cell growth (Probable).

1. Hirata, Y. et al. (1994) FEBS letters 356, 244-8. PMID: 7805847
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human CD157/BST1 Recombinant Antibody (A2), APC

    Flow CytoMetry

    Flow-cytometry using APC anti-human CD157 antibody. THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD157 monoclonal antibody (Catalog: HC654137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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