Anti-Human CD157 Reference Antibody (MEN1112, RUO) (HC654016)

Name: Anti-Human CD157 Reference Antibody (MEN1112, RUO)
Brand: abinScience
SKU: HC654016
Price: 370 USD
Availability: InStock

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Overview

Catalog No.	HC654016
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG
Clone ID	MEN1112
Biological activity	MEN1112 specifically binds to Bst1/CD157-positive cell lines with an EC 50 of 1 nM, and does not bind to Bst1/CD157-negative cell lines [1]. MEN1112 binds to recombinant Bst1/CD157 antigen with an EC 50 of 0.4 nM [1]. MEN1112 exhibits stronger high-affinity binding to both high- and low-affinity alleles of the CD16A Fc receptor on effector cells due to its defucosylated Fc domain [1]. MEN1112 induces slow internalization of the Bst1/CD157 antigen on target cells, a property that helps enhance its ADCC efficacy [1]. MEN1112 potently mediates antibody-dependent cell-mediated cytotoxicity (ADCC) against U937 acute myeloid leukemia (AML) cells, with an EC 50 of 30-140 pM [3]. MEN1112-mediated target cell lysis (20 h post-administration) is significantly enhanced upon the addition of 5% healthy donor serum to the co-culture system of healthy monocytes and autologous NK cells [3].
Expression system	Mammalian cells
Clonality	Monoclonal
Target	ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, Bone marrow stromal antigen 1, BST-1, ADP-ribosyl cyclase 2, CD157, Cyclic ADP-ribose hydrolase 2, BST1, cADPr hydrolase 2
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q10588
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	MEN1112
Background	ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2 (CD157/BST1) is a ~35 kDa protein. Catalyzes both the synthesis of cyclic ADP-beta-D-ribose (cADPR) from NAD(+), and its hydrolysis to ADP-D-ribose (ADPR). Cyclic ADPR is known to serve as an endogenous second messenger that elicits calcium release from intracellular stores, and thus regulates the mobilization of intracellular calcium (Probable). May be involved in pre-B-cell growth (Probable). 1. Hirata, Y. et al. (1994) FEBS letters 356, 244-8. PMID: 7805847
References	1. Aud D, et al. MEN1112, a Novel Humanized De-Fucosylated Monoclonal Antibody with High Affinity and Specificity for Bst1/CD157 Antigen and Enhanced CD16 Binding. Blood. 2014 Dec 6;124(21):3607. [HC654016] 2. Venditti A, et al. MEN1112/OBT357, an Anti Bst1/CD157 Humanized Antibody Inducing Acute Myelogenous Leukemia (AML) Blast Depletion in an Autologous Ex Vivo Assay: A Potential New Targeted Therapy for AML. Blood. 2015;126(23):788. [HC654016] 3. Krupka C, et al. Targeting AML Using an Fc-Engineered BST1/CD157 Monoclonal Antibody. Blood. 2014 Dec 6;124(21):987. [HC654016]
Note	For research use only. Not suitable for clinical or therapeutic use.