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Обзор
Каталожный номерHV234014
Описание
Anti-Human CD169/SIGLEC1 Polyclonal Antibody (HV234014) is a rabbit polyclonal antibody detecting CD169/SIGLEC1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human CD169/SIGLEC1 (Gly34-Gly240).
Цель Siglec-1, CD169, SIGLEC1, SN, Sialic acid-binding Ig-like lectin 1, Sialoadhesin
Очистка Purified by antigen affinity column.
Номер доступа Q9BZZ2
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Sialoadhesin (CD169/SIGLEC1) is a ~182 kDa protein. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Plays a crucial role in limiting bacterial dissemination by engaging sialylated bacteria to promote effective phagocytosis and antigen presentation for the adaptive immune response. Mediates the uptake of various enveloped viruses via sialic acid recognition and subsequently induces the formation of intracellular compartments filled with virions (VCCs). In turn, enhances macrophage-to-T-cell transmission of several viruses including HIV-1 or SARS-CoV-2. Acts as an endocytic receptor mediating clathrin dependent endocytosis.

1. Jones, C. et al. (2003) Molecular microbiology 49, 1213-25. PMID: 12940982
2. Benet, S. et al. (2021) Journal of extracellular vesicles 10, e12046. PMID: 33489013
3. Hammonds, JE. et al. (2017) PLoS pathogens 13, e1006181. PMID: 28129379
4. Perez-Zsolt, D. et al. (2021) Cellular & molecular immunology 18, 2676-2678. PMID: 34782760
5. Zheng, Q. et al. (2015) Cell research 25, 1121-36. PMID: 26358190
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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