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Human CD169/SIGLEC1 Recombinant Protein (C-Fc) (HV234021)

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Human CD169/SIGLEC1 Recombinant Protein (C-Fc)

Human CD169/SIGLEC1 Recombinant Protein (C-Fc)

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Datasheet
SDS

Overview

Catalog No.	HV234021
Description	Recombinant Human CD169/SIGLEC1 Protein, C-Fc (HV234021) expressed in Mammalian Cells, spanning Met1-Gln1641. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	Q9BZZ2
Protein length	Met1-Gln1641
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Sialoadhesin, Sialic acid-binding Ig-like lectin 1, Siglec-1, CD169, SIGLEC1, SN
Background	Sialoadhesin (CD169/SIGLEC1) is a ~182 kDa protein. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Plays a crucial role in limiting bacterial dissemination by engaging sialylated bacteria to promote effective phagocytosis and antigen presentation for the adaptive immune response. Mediates the uptake of various enveloped viruses via sialic acid recognition and subsequently induces the formation of intracellular compartments filled with virions (VCCs). In turn, enhances macrophage-to-T-cell transmission of several viruses including HIV-1 or SARS-CoV-2. Acts as an endocytic receptor mediating clathrin dependent endocytosis. 1. Jones, C. et al. (2003) Molecular microbiology 49, 1213-25. PMID: 12940982 2. Benet, S. et al. (2021) Journal of extracellular vesicles 10, e12046. PMID: 33489013 3. Hammonds, JE. et al. (2017) PLoS pathogens 13, e1006181. PMID: 28129379 4. Perez-Zsolt, D. et al. (2021) Cellular & molecular immunology 18, 2676-2678. PMID: 34782760 5. Zheng, Q. et al. (2015) Cell research 25, 1121-36. PMID: 26358190
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human CD169/SIGLEC1 Protein, C-Fc

References

HV234021-SDS-PAGE-1.jpg

Datasheet

Human CD169/SIGLEC1 Recombinant Protein (C-Fc).pdf

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Formula

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Formula

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Stock Solution

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Working Solution

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Human CD169/SIGLEC1 Recombinant Protein (C-Fc)