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Anti-Human L1CAM Recombinant Antibody (OV52.24) (HB159107)

Anti-Human L1CAM Recombinant Antibody (OV52.24)
Anti-Human L1CAM Recombinant Antibody (OV52.24)
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Aperçu
Numéro de catalogueHB159107
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de cloneOV52.24
Cible CAML1, Neural cell adhesion molecule L1, NCAM-L1, N-CAM-L1, CD171, MIC5, L1CAM
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P32004
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Neural cell adhesion molecule L1 (CD171/L1CAM) is a ~140 kDa protein. Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

Reférences 1. Chen K, et al. mRNA Vaccines Against SARS-CoV-2 Variants Delivered by Lipid Nanoparticles Based on Novel Ionizable Lipids. Adv Funct Mater. 2022 Sep 26;32(39):2204692. [HB159107]
Note For research use only.
Images
  • Anti-Human L1CAM Recombinant Antibody (OV52.24)

    Flow Cytometry

    Flow-cytometry using anti-human CD171 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD171 antibody monoclonal antibody (Catalog # HB159107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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