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Anti-Human CD171/L1CAM Recombinant Antibody (OV52.24), APC (HB159137)

Anti-Human CD171/L1CAM Recombinant Antibody (OV52.24), APC
Anti-Human CD171/L1CAM Recombinant Antibody (OV52.24), APC
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Overview
Catalog No.HB159137
Description
Anti-Human CD171/L1CAM Antibody (OV52.24), APC [OV52.24] (HB159137) is a mouse monoclonal antibody detecting CD171 in FCM. Suitable for Human.
Highlights
  • Flow Cytometry — Validated for flow cytometry applications.
  • Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG1, kappa
Clone IDOV52.24
ConjugationAPC
Clonality Monoclonal
Target CAML1, Neural cell adhesion molecule L1, NCAM-L1, N-CAM-L1, CD171, MIC5, L1CAM
Endotoxin level Please contact with the lab for this information.
Accession P32004
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Neural cell adhesion molecule L1 (CD171/L1CAM) is a ~140 kDa protein. Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human CD171/L1CAM Recombinant Antibody (OV52.24), APC

    Flow CytoMetry

    Flow-cytometry using APC anti-human CD171 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD171 monoclonal antibody (Catalog: HB159137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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