Anti-Human Integrin beta 2/CD18 Recombinant Antibody (SAA0815) (HY378107)

Name: Anti-Human Integrin beta 2/CD18 Recombinant Antibody (SAA0815)
Brand: abinScience
SKU: HY378107
Price: 196 USD
Availability: InStock

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Numéro de catalogue	HY378107
Reactivité des espèces	Human
Applications	ELISA, FCM, IHC, WB
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG1
ID de clone	SAA0815
Applications testées	FCM
Cible	CD18, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Complement receptor C3 subunit beta, ITGB2, Integrin beta-2, MFI7
Niveau d'endotoxines	< 10 EU/mg
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P05107
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte	Integrin beta-2 (CD18/ITGB2) is a ~84 kDa protein. Integrin ITGAL:ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3. Integrin ITGAL:ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL. Integrins ITGAM:ITGB2 and ITGAX:ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX:ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM:ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. 1. de Fougerolles, AR. et al. (1991) The Journal of experimental medicine 174, 253-67. PMID: 1676048 2. Kristóf, E. et al. (2013) Apoptosis : an international journal on programmed cell death 18, 1235-51. PMID: 23775590 3. Lacouture, C. et al. (2024) Nature communications 15, 407. PMID: 38195629 4. Swaim, CD. et al. (2017) Molecular cell 68, 581-590.e5. PMID: 29100055 5. Van der Vieren, M. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 163, 1984-90. PMID: 10438935 6. Van der Vieren, M. et al. (1995) Immunity 3, 683-90. PMID: 8777714 7. Grayson, MH. et al. (1998) The Journal of experimental medicine 188, 2187-91. PMID: 9841932 8. Barber, DF. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 3653-9. PMID: 15356110 9. Ostermann, G. et al. (2002) Nature immunology 3, 151-8. PMID: 11812992 10. Bai, M. et al. (2017) Blood 130, 2092-2100. PMID: 28807980
Note	For research use only.

Images

Flow-cytometry

Flow-cytometry using anti- human CD18 antibody.
Human U-937cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD18 antibody monoclonal antibody (Catalog # HY378107 , Red Histogram) at a concentration of 20 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti- human CD18 antibody.
Human Jurkat cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD18 antibody monoclonal antibody (Catalog # HY378107 , Red Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

SEC-HPLC detection for Anti-Human CD18/ITGB2 Antibody (ABS0955).
Flow CytoMetry

Flow-cytometry using anti-human CD18 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD18 monoclonal antibody (Catalog: HY378107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.