Anti-Human Integrin beta 2/CD18 Reference Antibody (LDP-01, RUO) (HY378023)

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50ug
100ug
1mg

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Datasheet
SDS

Overview

Catalog No.

HY378023

Description

Anti-CD18/ITGB2 (LDP-01) (HY378023) is a research-grade recombinant antibody targeting CD18. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

Species reactivity

Human

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Host species

Humanized

Isotype

IgG-nd

Clone ID

LDP-01

Expression system

Mammalian Cells

Clonality

Monoclonal

Target

Complement receptor C3 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, Integrin beta-2, MFI7, CD18, ITGB2

Endotoxin level

Please contact the lab for this information.

Purity

>95% purity as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P05107

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.

Background

Integrin beta-2 (CD18/ITGB2) is a ~84 kDa protein. Integrin ITGAL:ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3. Integrin ITGAL:ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL. Integrins ITGAM:ITGB2 and ITGAX:ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX:ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM:ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain.

1. de Fougerolles, AR. et al. (1991) The Journal of experimental medicine 174, 253-67. PMID: 1676048

2. Kristóf, E. et al. (2013) Apoptosis : an international journal on programmed cell death 18, 1235-51. PMID: 23775590

3. Lacouture, C. et al. (2024) Nature communications 15, 407. PMID: 38195629

4. Swaim, CD. et al. (2017) Molecular cell 68, 581-590.e5. PMID: 29100055

5. Van der Vieren, M. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 163, 1984-90. PMID: 10438935

6. Van der Vieren, M. et al. (1995) Immunity 3, 683-90. PMID: 8777714

7. Grayson, MH. et al. (1998) The Journal of experimental medicine 188, 2187-91. PMID: 9841932

8. Barber, DF. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 3653-9. PMID: 15356110

9. Ostermann, G. et al. (2002) Nature immunology 3, 151-8. PMID: 11812992

10. Bai, M. et al. (2017) Blood 130, 2092-2100. PMID: 28807980

Note

For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-CD18/ITGB2 (LDP-01).

References

Datasheet

Anti-Human Integrin beta 2/CD18 Reference Antibody (LDP-01, RUO).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human Integrin beta 2/CD18 Reference Antibody (LDP-01, RUO) (HY378023)

Overview

Images

References

Datasheet

SDS

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