Anti-Human TRAIL R2/TNFRSF10B Recombinant Antibody (SAA1413) (HT348307)

Name: Anti-Human TRAIL R2/TNFRSF10B Recombinant Antibody (SAA1413)
Brand: abinScience
SKU: HT348307
Price: 191 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HT348307
Reactivité des espèces	Human
Applications	FCM
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG2a, lambda
ID de clone	SAA1413
Applications testées	FCM
Cible	CD262, DR5, Death receptor 5, KILLER, TNF-related apoptosis-inducing ligand receptor 2, TNFRSF10B, TRAIL receptor 2, TRAIL-R2, TRAILR2, TRICK2, Tumor necrosis factor receptor superfamily member 10B, ZTNFR9
Niveau d'endotoxines	< 10 EU/mg
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	O14763
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte	Tumor necrosis factor receptor superfamily member 10B (CD262/TRAIL-R2) is a ~47 kDa protein. Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. 1. Hymowitz, SG. et al. (1999) Molecular cell 4, 563-71. PMID: 10549288
Note	For research use only.

Images

Flow-cytometry

Flow-cytometry using anti-human CD262 antibody.CD262 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD262 antibody monoclonal antibody (Catalog # HT348307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD262 antibody.HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD262 antibody monoclonal antibody (Catalog # HT348307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD262 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD262 antibody monoclonal antibody (Catalog # HT348307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

SEC-HPLC detection for Anti-Human CD262/TNFRSF10B/TRAIL-R2 Antibody (ABS1916).
Flow CytoMetry

Flow-cytometry using anti-human CD262 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD262 monoclonal antibody (Catalog: HT348307) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.