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| カタログ番号 | HB004033 | ||||||
|---|---|---|---|---|---|---|---|
| 種反応性 | Human | ||||||
| アプリケーション | ELISA, WB, FCM | ||||||
| 宿主種 | Mouse | ||||||
| クローン性 | Monoclonal | ||||||
| アイソタイプ | VHH-mFc (IgG2a) | ||||||
| クローンID | SAA2179 | ||||||
| テスト済みアプリケーション | FCM | ||||||
| ターゲット | Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1 | ||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | P16284 | ||||||
| RRID | Anti-Human CD31/PECAM1 Antibody (SAA2179) (abinScience Cat# HB004033, RRID:AB_3729544) |
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| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 背景 | Platelet endothelial cell adhesion molecule (CD31/PECAM1) is a ~82 kDa protein. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. 1. Sachs, UJ. et al. (2007) The Journal of biological chemistry 282, 23603-12. PMID: 17580308 2. Dasgupta, B. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 5041-51. PMID: 19342684 3. Hu, M. et al. (2016) Scientific reports 6, 38655. PMID: 27958302 4. Brown, S. et al. (2002) Nature 418, 200-3. PMID: 12110892 5. Yeh, JC. et al. (2008) Biochemistry 47, 9029-39. PMID: 18672896 | ||||||
| 注意事項 | For research use only. | ||||||
| Isotype Control | Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507) |
Flow-cytometry using anti-human CD31 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD31 monoclonal antibody (Catalog HB004033, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD31 antibody. THP-1 cells were stained with an irrelevant antibody (Green Histogram) or an anti-human CD31 antibody monoclonal antibody (Catalog # HB004033 , Blue Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (AntibodySystem: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human CD31/PECAM1 Antibody (SAA2179)
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